Program
Main Lecture topics
- The Fourier Transform in 1D - 2D - 3D
- Properties of the Fourier Transform
- Specimen Preparation for single-particle cryo-EM
- (Automatic) Particle Selection
- Using the IMAGIC software system
- Filtering and Data Normalization
- Optimizing the EM data collection (CTF)
- CTF correction
- Alignment using Correlation Functions
- Multi Reference Alignments
- Multivariate Statistics and Classification
- Three Dimensional Reconstruction
- Angular Reconstitution
- Projection Matching
- Parallel Processing
- Alignment Refinements
- 3D Refinements
- 3D Fitting known X-ray Structures
- 3D resolution criteria
- Refining towards different 3D structures simultaneously
- Advanced topics including Solvent Flattening and astigmatic data processing
Daily School Schedule
- Two or three morning lectures (8:30 am - 10:30 am)
- Hands-on single particle analysis.
- Lunch (1 pm - 2 pm)
- Open Afternoons (2 pm - 5 pm)
- 5 pm - 8 pm: Evening lectures / Hands-on processing
- Dinner 8 - 9 pm
- Open program after dinner
Leisure Programme
- Swimming Pool
- Visit to the historic center district
- Rafting
- Two Churasco Evenings
- Capoeira night