Santa Rita School
for
Single Particle Cryo-EM
18-30 August 2005
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Main Lecture topics
- The Fourier Transform in 1D - 2D - 3D
- Properties of the Fourier Transform
- Specimen Preparation for single-particle cryo-EM
- (Automatic) Particle Selection
- Using the IMAGIC software system
- Filtering and Data Normalization
- Optimizing the EM data collection (CTF)
- CTF correction
- Alignment using Correlation Functions
- Multi Reference Alignments
- Multivariate Statistics and Classification
- Three Dimensional Reconstruction
- Angular Reconstitution
- Projection Matching
- Parallel processing on computer farms
- Alignment Refinements
- 3D Refinements
- 3D Rendering
- 3D Fitting known X-ray Structures
- 3D resolution criteria
- Refining towards different 3D structures simultaneously
- Advanced topics (including Solvent Flattening)
Lecturers
- Bruno Klaholz
- Holger Stark
- Michael Schatz
- Marin van Heel
- Ralf Schmidt
- Igor Polikarpov
- Elena Orlova
- Ardan Patwardhan
Daily School Schedule
- Two or three morning lectures (8:30 am - 10:30 am)
- Hands on single particle analysis.
- Lunch (1 pm - 2 pm)
- Afternoons open (2 pm - 5 pm)
- 5 pm - 8 pm: Evening lectures / Hands-on processing
- Dinner 8 - 9 pm
- Open program after dinner
Leisure Programme
- White water rafting
- Gloria Swimming Pool
- Mountain biking
- Horseback riding
- Two Churasco Evenings
- The waterfalls of Santa Rita
- Canoeing on the Mogi Guass
