School Programme
Main Lecture Topics
- • The Fourier transform in 1D - 2D - 3D
- • Properties of the Fourier transform
- • Specimen preparation for single-particle cryo-EM
- • (Automatic) Particle selection
- • Optimizing the EM data collection
- • CTF correction
- • Filtering and data normalization
- • Alignment using correlation functions
- • Multi-reference alignments
- • Multivariate statistics and classification
- • Angular reconstitution
- • Three-dimensional reconstruction
- • Projection matching
- • Alignment refinements
- • 3D refinements
- • 3D resolution criteria
- • 3D fitting of known X-ray structures (Chimera, Coot)
- • Refining towards different 3D structures simultaneously ("4D" analysis)
- • Using image processing software (IMAGIC, Frealign)
- • Parallel processing
Participant's Talks
- Presenting the own project
Daily School Schedule
- • Two or three morning lectures
- • Hands-on single particle analysis
- • Lunch
- • Open afternoons / Time for additional workshops
- • Evening lectures / Hands-on processing
- • Dinner
- • Open program after dinner
Leisure Programme
- • Swimming Pool
- • Tours and trips
- • Rafting, horse riding, sports
- • Churasco evenings
- • Caipirinha night
- • More...